Duet Yamaha Valentino Rossi & Jorge Lorenzo menguasai jalannya latihan bebas ketiga MotoGP Assen dengan menduduki posisi satu & kedua diikuti oleh duet Ducati Andrea Iannone & Andrea Dovizioso.
Sementara duet Repsol Honda Marc Marquez dan Dani Pedrosa hanya menduduki posisi keenam dan ketujuh dalam latihan bebas ketiga MotoGP Assen.
1. Valentino Rossi ITA Movistar Yamaha MotoGP (YZR-M1) 1m 33.167s [Lap 18/18] 309km/h (Top Speed)
2. Jorge Lorenzo ESP Movistar Yamaha MotoGP (YZR-M1) 1m 33.275s +0.108s [17/18] 310km/h
3. Andrea Iannone ITA Ducati Team (Desmosedici GP15) 1m 33.292s +0.125s [16/16] 320km/h
4. Andrea Dovizioso ITA Ducati Team (Desmosedici GP15) 1m 33.356s +0.189s [15/15] 319km/h
5. Bradley Smith GBR Monster Yamaha Tech 3 (YZR-M1) 1m 33.387s +0.220s [17/18] 313km/h
6. Marc Marquez ESP Repsol Honda Team (RC213V) 1m 33.477s +0.310s [10/16] 314km/h
7. Dani Pedrosa ESP Repsol Honda Team (RC213V) 1m 33.508s +0.341s [16/16] 315km/h
8. Maverick Viñales ESP Team Suzuki Ecstar (GSX-RR)* 1m 33.526s +0.359s [13/17] 306km/h
9. Pol Espargaro ESP Monster Yamaha Tech 3 (YZR-M1) 1m 33.660s +0.493s [14/16] 315km/h
10. Cal Crutchlow GBR CWM LCR Honda (RC213V) 1m 33.744s +0.577s [14/14] 313km/h
11. Danilo Petrucci ITA Octo Pramac Racing (Desmosedici GP14.1) 1m 33.803s +0.636s [16/18] 311km/h
12. Aleix Espargaro ESP Team Suzuki Ecstar (GSX-RR) 1m 33.954s +0.787s [15/16] 301km/h
13. Stefan Bradl GER Athina Forward Racing (Forward Yamaha) 1m 34.195s +1.028s [16/16] 307km/h
14. Scott Redding GBR Estrella Galicia 0,0 Marc VDS (RC213V) 1m 34.223s +1.056s [16/17] 305km/h
15. Hector Barbera ESP Avintia Racing (Desmosedici GP14 Open) 1m 34.406s +1.239s [16/17] 315km/h
16. Yonny Hernandez COL Octo Pramac Racing (Desmosedici GP14.2) 1m 34.440s +1.273s [14/15] 310km/h
17. Alvaro Bautista ESP Factory Aprilia Gresini (RS-GP) 1m 34.668s +1.501s [14/16] 305km/h
18. Mike Di Meglio FRA Avintia Racing (Desmosedici GP14 Open) 1m 35.013s +1.846s [13/15] 310km/h
19. Eugene Laverty IRL Aspar MotoGP Team (RC213V-RS)* 1m 35.026s +1.859s [15/17] 305km/h
20. Nicky Hayden USA Aspar MotoGP Team (RC213V-RS) 1m 35.177s +2.010s [14/15] 304km/h
21. Jack Miller AUS CWM LCR Honda (RC213V-RS)* 1m 35.420s +2.253s [14/15] 303km/h
22. Loris Baz FRA Athina Forward Racing (Forward Yamaha)* 1m 36.030s +2.863s [6/7] 300km/h
23. Alex De Angelis RSM E-Motion IodaRacing (ART) 1m 36.298s +3.131s [12/13] 295km/h
24. Marco Melandri ITA Factory Aprilia Gresini (RS-GP) 1m 37.389s +4.222s [14/14] 305km/h